How Protein Sequencing Methods Can Revolutionize Biological Research

Recently a study conducted by the University of Texas revealed a new way of protein sequencing far more sensitive than the present method. You can now identify individual proteins rather than millions being determined concurrently.

The breakthrough could significantly impact biomedical research by making it easier to detect new biomarkers for disease and better understand how healthy tissue operates.

This article will discuss in detail how protein sequencing methods can impact biological research. 

What is Protein Synthesis?


Protein synthesis is a necessary process that occurs regularly within cells. The procedure is used to generate new proteins, which are then employed by the body for various critical purposes. The procedure consists of two stages: transcription and translation, with the requirement for processing. 

Transcription is the process through which genetic information is transferred from DNA to mRNA via initiation, elongation, and termination. The freshly formed mRNA strand then exits the nucleus and connects to a ribosome in the cytoplasm. 

This is the point at which translation begins. The genetic data is read during this stage, prompting tRNA to carry the correct sequence of amino acids to the ribosome, resulting in the formation of a polypeptide chain. Finally, you may process the polypeptide chain to form the completed protein molecule.

How Protein Sequencing Methods Can Revolutionize Biological Research

The Science Behind Protein Sequencing


An isolated polypeptide or glutamate is a linearly ordered sequence of amino acids. The hydroxyl (C) end constantly influences a protein’s fundamental structure’s carboxylate (N) end. Depending on the method utilized, Peptide Synthesis can be sequenced or inferred from its DNA chronology. Protein sequencing is finding and examining the fatty acid phylogeny of proteins. 

The same techniques as antibody protein sequencing services were employed for Edman degradation, spectroscopy, and bioinformatics extrapolation from the original DNA or mRNA sequence. 

Letters are widely used to signify the amino acid order in a secondary structure from the amino-terminal to the carboxyl-terminal end, depending on the number of letters in the code. Each cytosine could have a code of one to three letters. There are twenty different amino acids known to exist in nature. 

Protein Sequencing techniques


1.Edman Degradation


Edman degradation is a protein purification method that eliminates one molecule of the protein chain’s carboxyl terminus. To address the challenge of hydrolyzing conditions that were adverse to the protein’s functionality, Pehr Edman created a novel method of flagging and splicing the polypeptide. 

He invented a method for deleting only one residue from a sequence while leaving the remainder of the sequence intact. By reacting the Hydroxy substance with the N-terminal of another phenylthiocarbamoyl derivative, a phenylthiocarbamoyl derivative was created. Under less acidic conditions, the N-terminal is cleaved, producing a phenylthiohydantoin Glp-1 acid circular polymer.


2. Mass Spectrometry


The use of mass spectroscopy to decipher proteins is an increasingly prominent proteomic technology that has gained widespread acceptance. When enzymes and other substances degrade proteins, thin-layer chromatography can separate peptides.

Each fraction containing ten distinct peptides is then examined using an automated multi-analyzer system that employs collision-activated dissociation. 


Protein Sequencing Methods are Positively Impacting Biological Research

A team of researchers from The University of Texas at Austin has revealed a new method for sequencing proteins far more sensitive than existing technologies, recognizing individual protein molecules rather than millions of molecules simultaneously. The breakthrough could significantly influence biomedical research, making it easier to discover new biomarkers for cancer and other disorders and improve our understanding of how healthy cells behave.

Prof Marcotte and colleagues proposed extending the methodologies of next-generation gene sequencing to protein sequencing more than six years ago. Next-generation gene sequencing refers to a group of techniques that have allowed sequencing the complete genome of any living thing to be fast, accurate, and affordable, thereby advancing biological research and enabling at-home genetic testing for ancestry and disease for the rest of us.

Similar to how previous developments offered quick and complete information on thousands of genes that influence human health, the new technique delivers quick and comprehensive information about tens of thousands of proteins that play a role in healthy functioning or disease. 

Cells create proteins and other chemicals that act as distinct biomarkers, similar to fingerprints, in numerous conditions such as cancer, heart failure, Alzheimer’s, and diabetes. Better identification of these biomarkers might aid researchers in understanding the etiology of illnesses or give patients an earlier, more accurate diagnosis. 

The current laboratory standard for sequencing proteins, mass spectrometry, is insensitive for many applications, detecting a protein only if there are around a million copies of it. It also has a “poor throughput,” which means it can only see a few thousand different protein types in a single sample.



We expect these ground-breaking technologies to penetrate the market, particularly in academic research, the pharmaceutical industry, and clinical diagnostics. Using these technologies, we will be able to uncover the diversity of the proteome, study how the proteins communicate and interact, and provide new insights into how life works at the molecular level.

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